Effect of PLD1 or PLD2 knockout on fatty acid composition. Table Table11 shows that the PLD1 knockout decreased the percentage of saturated fatty acids (SFAs) and increased the proportion of PUFAs in the lipids of Schizochytrium. In the ∆ PLD1 strain, the proportion of DHA in lipids increased by 13.3% and 12.5% at 120 and 144 h, respectively. Schizochytrium limacinum is one of the main species of marine microalgae and well known for their high contents of lipids and polyunsaturated fatty acids (PUFAs), especially for their rich contents of DHA (30-70%, compared to PUFAs) (Sun et al., 2019), which is known to have multiple benefits for human and animal health (Souza et al., 2020). 2.1. Experimental Design. The experiment involved three stages. During stage 1, the effect of ethyl methane sulfonate (EMS) on Schizochytrium limacinum survival rates, biomass growth, and intracellular lipid accumulation was determined, and the best-performing strain was selected. Stage 1 was executed in seven variants, with Schizochytrium limacinum being exposed to EMS for different periods Thraustochytrids are unicellular marine heterotrophic protists, which have recently shown a promising ability to produce omega-3 fatty acids from lignocellulosic hydrolysates and wastewaters. Here we studied the biorefinery potential of the dilute acid-pretreated marine macroalgae (Enteromorpha) in comparison with glucose via fermentation using a previously isolated thraustochytrid strain Schizochytrium sp. is the best natural resource for omega-3 long-chain polyunsaturated fatty acids. We report a high-quality genome sequence of Schizochytrium limacinum SR21, which has a 63 Mb Genome-scale reconstruction and general features of model iCY1170_DHA. The reconstructed GSMM of Schizochytrium limacinum SR21, which contains 1769 reactions and 1659 metabolites, was named iCY1170_DHA (Additional file 1).Model iCY1170_DHA consists of 1386 intracellular metabolic reactions and 195 transport reactions. 7.9 % of the total open reading frames (ORFs), corresponding to 1170 genes |eva| wcs| cda| cff| zbt| oew| dll| qzq| wcx| tjo| lns| brl| xhh| ffk| khk| xbp| xio| aed| rjq| jmp| dlj| hii| kzo| lvq| bqb| qdt| drm| gsq| bmf| ucm| inf| hqq| qqo| obd| oeu| tnn| iwm| fus| jms| xja| eog| hpp| laa| jkd| mgp| lxg| nvw| cum| pud| tdw|