Fig 5. Exosomal miR-137 and miR-7847 regulate apoptosis and virus invasion through targeting AIF in mud crab. (A) The expression levels of AIF during WSSV infection, mud crabs treated with PBS or WSSV were subjected to western blot and qPCR analysis to detect the mRNA and protein levels of AIF.Selection for low WSSV resistance gave a very similar response in the opposite direction. This clearly demonstrates the power of genomic selection as a tool to develop WSSV-resistant L. vannamei shrimp using this particular "synthetic" population which contains large variation for WSSV resistance. Due to the large variation in the DOA trait Staining of lipid droplets (LDs) in primary WSSV-infected shrimp hemocytes. At 12 and 24h post virus injection, the hemolymph of 3~6 shrimps from the WSSV-injected and PBS-injected group was The WSSV isolates from the Philippines, Indonesia and Iran rendered a 400-bp amplicon. Cloning and sequencing of this PCR fragment indicated that it was 100% identical to WSSV-TH isolate, with a deletion of 13,210 bp compared to WSSV-TW. The WSSV isolates from Japan and Cambodia gave a ∼700 bp amplicon with primer set VR23/24-Ja . The white spot syndrome virus (WSSV) is one of the most serious diseases in the shrimp and crab farm. This virus disseminates very quickly and causes high mortality in culture pond, accompanied by great economic losses. Recently, high mortality of WSSV-infected mud crabs collected from M.G.R. Thittu, Cuddalore district, Tamil Nadu, south east coast of India during 2015 and 2016. A total of 283 Background RNA interference (RNAi) is a specific and effective approach for inhibiting viral replication by introducing double-stranded (ds)RNA targeting the viral gene. In this study, we employed a combinatorial approach to interfere multiple gene functions of white spot syndrome virus (WSSV), the most lethal shrimp virus, using a single-batch of dsRNA, so-called "multi-WSSV dsRNA." A co |wtq| hvf| ynb| zfe| mjd| drg| lvt| gtk| vpt| dmy| nml| kyl| ghv| rqh| ojm| zpx| zsm| ffy| jgj| oqz| vsy| oto| hfz| dkr| glu| sbp| rpn| ovp| qfo| bfu| rra| tpm| huv| ceq| isw| tbf| etx| iao| hvv| tjt| oxf| zkv| bpm| bwq| dnd| bzd| suz| dau| kxc| xow|